RFP Antibody MA5-15257 from Thermo Fisher Scientific

Product Specs
ItemRFP Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMA5-15257
IsotypeIgG1
Quantity100 µg
Conjugate/TagUnconjugated
TargetRFP
ApplicationsELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
FormatPBS with no preservative; pH 7.2
HostMouse
ReactivityTag
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
CloneRF5R
ClonalityMonoclonal
Concentration1 mg/mL
ImmunogenRFP from the Discosoma sea anemone N-terminal peptide-KLH conjugated
PurityProtein A
Antibody TypePrimary
StorageStore at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) NRF2/ARE mediated antioxidant response to glaucoma: role of glia and retinal ganglion cellsActa Neuropathologica CommunicationsOctober 24, 2023Sarah Naguib, Jon R. Backstrom, Elisabeth Artis, Purnima Ghose, Amy Stahl, Rachael Hardin, Ameer A. Haider, John Ang, David J. Calkins, Tonia S. Rexfor IHC Nrf2,Abcam,"137,550",Rabbit,1:1000,1:200 pNrf2,ThermoFisher,PA5-67520,Rabbit,1:1000,1:200 b-actin,Cell Signaling,E4D9Z,Mouse,1:1000,N/A RFP (for tdTomato),ThermoFisher,MA5-15257,Mouse,1:200,1:200 Prdx6,Abcam,"133,348",Rabbit,1:1000,N/A Gpx1,ThermoFisher,PA5-26323,Rabbit,1:500,N/A SOD3,Abcam,"80,946",Rabbit,1:1000,N/A b-tubulin,Sigma,T8678,Mouse,N/A,1:300 Antibodies

(2) Bumetanide induces post-traumatic microglia–interneuron contact to promote neurogenesis and recoveryBrainOctober 3, 2023Marine Tessier, Marta Saez Garcia, Emmanuelle Goubert, Edith Blasco, Amandine Consumi, Benoit Dehapiot, Li Tian, Florence Molinari, Jerome Laurin, François Guillemot, et al.anti-GFAP (MAB360, Merck Millipore, 1:500), mouse anti-BDNF antibody (MAB#918), mouse anti-RFP antibody (MA5-15257, ThermoFisher Scientific, 1:500), chicken anti-GFP antibody (AB_2307313, Avès Labs, 1:300) as well as

(3) Combinatorial design of nanoparticles for pulmonary mRNA delivery and genome editingNature BiotechnologyOctober 1, 2023Bowen Li, Rajith Singh Manan, Shun-Qing Liang, Akiva Gordon, Allen Jiang, Andrew Varley, Guangping Gao, Robert Langer, Wen Xue, Daniel Andersonanti-CCSP antibody (Santa Cruz, Cat. # SC-25555, 1:2000) and an anti-tdTomato antibody (ThermoFisher, Cat. #MA5-15257, 1:300). The sections were then incubated for one hour at room temperature with Alexa Fluor 488 Donkey

(4) MIRO‐1 interacts with VDAC‐1 to regulate mitochondrial membrane potential in Caenorhabditis elegansEMBO reportsAugust 3, 2023Xuecong Ren, Hengda Zhou, Yujie Sun, Hongying Fu, Yu Ran, Bing Yang, Fan Yang, Mikael Bjorklund, Suhong Xu(1:2,500, MBL598, Japan), anti‐VDAC‐1 (1:2,000, Proteinteck, 55259, USA), anti‐RFP (1:1,000, Thermofisher, MA5‐15257, USA), anti‐streptavadin (1:1,000, Bioss, bs‐0437P, China), anti‐biotin (1:1,000, Bioss, bs‐0311R, China),

(5) Chronic Alcohol Drinking Drives Sex-Specific Differences in Affective Behavior and Medial Prefrontal Cortex Activity in CRF1:Cre:tdTomato Transgenic RatseneuroJuly 6, 2023Sema G. Quadir, Gillian M. Arleth, Meredith G. Cone, Margaret W. High, Maria C. Ramage, Devin P. Effinger, Maria Echeveste Sanchez, Melissa A. Hermanprimary antibody (1:3000, Millipore Sigma ABE457) and mouse anti-RFP primary antibody (1:500, Invitrogen MA5-15257) in blocking solution. Slices were then washed with 0.1% Tween 20 in Tris-buffered saline (TNT) before

(6) Cell detoxification of secondary metabolites by P4-ATPase-mediated vesicle transporteLifeJuly 4, 2023Yujie Li, Hui Ren, Fanlong Wang, Jianjun Chen, Lian Ma, Yang Chen, Xianbi Li, Yanhua Fan, Dan Jin, Lei Hou, et al.were analyzed by immunoblotting with anti-Ub (ubiquitin, PTM-1107, PTM-BIO) and anti-RFP antibodies (MA5-15257, Thermo Fisher Scientific). Southern blot Southern blot was performed according to DIG High Prime

(7) Interferon signaling promotes tolerance to chromosomal instability during metastatic evolution in renal cancerNature cancerJuly 1, 2023Perelli L, Carbone F, Zhang L, Huang JK, Le C, Khan H, Citron F, Del Poggetto E, Gutschner T, Tomihara H, et al.immunofluorescence were performed as previously described45. Antibodies list: RFP (ThermoFisher, catalog no. MA5-15257, 1:100 dilution), GFP (Abcam, catalog no. 13970, 1:100 dilution), Vimentin (Abcam, catalog no. ab8978,

(8) Viral vector gene delivery of the novel chaperone protein SRCP1 to modify insoluble protein in in vitro and in vivo models of ALSGene therapyJune 1, 2023Luecke IW, Lin G, Santarriaga S, Scaglione KM, Ebert AD.incubated overnight at room temperature in primary antibody solution (mouse anti-RFP (ThermoFisher MA5-15257, 1:1000) in PBS/0.3% Triton-TX, and NGS). Sections were then incubated in secondary antibody (goat anti-mouse

(9) Hyperphosphorylated PTEN exerts oncogenic propertiesNature communicationsMay 24, 2023van Ree JH, Jeganathan KB, Fierro Velasco RO, Zhang C, Can I, Hamada M, Li H, Baker DJ, van Deursen JM.C-terminal human PTEN (Invitrogen Cat. #51-2400) or 1 µg Red Fluorescent Protein (control; Invitrogen Cat. #MA5-15257) were added. After 45 min, 20 µl 50% protein G agarose suspension (in PBS) was added and after 45 min

(10) Dedifferentiation maintains melanocyte stem cells in a dynamic nicheNatureApril 27, 2023Qi Sun, Wendy Lee, Hai Hu, Tatsuya Ogawa, Sophie De Leon, Ioanna Katehis, Chae Ho Lim, Makoto Takeo, Michael Cammer, M. Mark Taketo, et al.rabbit anti-Tomato (1:1,000; Rockland, 600–401–379); mouse anti-Tomato (RF5R) (1:500; Thermo Fisher, MA5–15257); rabbit anti-Tyrp1 (1:100; Sigma-Aldrich, SAB2102617); mouse anti-E-cadherin (1:100; BD Transduction,

(11) A circuit for secretion‐coupled cellular autonomy in multicellular eukaryotic cellsMolecular Systems BiologyApril 12, 2023Lingxia Qiao, Saptarshi Sinha, Amer Ali Abd El‐Hafeez, I‐Chung Lo, Krishna K Midde, Tony Ngo, Nicolas Aznar, Inmaculada Lopez‐Sanchez, Vijay Gupta, Marilyn G Farquhar, et al.Invitrogen (Thermo Scientific)",Catalog # MA5‐15256 Anti‐RFP,Invitrogen (Thermo Scientific),Catalog # MA5‐15257 Anti‐GIV coiled coil antibody,Millipore (Sigma),ABT80 "Goat anti‐Rabbit IgG, Alexa Fluor 594 conjugated",Thermo Fisher Scientific

(12) Retroviral lineage analysis reveals dual contribution from ectodermal placodes and neural crest cells to avian olfactory sensory and GnRH neuronsNatural SciencesOctober 31, 2022Alison Koontz, Hugo A. Urrutia, Marianne E. Bronner1:100); for anti-RFP(rabbit 1:100) (MBL Cat#PM005); for anti-RFP IgG1 (Thermofisher Scientic Catalog # MA5-15257 1:100); for anti-GFP (1:100); DAPI (1:1000). Secondary Alexa Abs (Molecular Probes) were used 1:1000.

(13) Molecular mechanism of Afadin substrate recruitment to the receptor phosphatase PTPRK via its pseudophosphatase domaineLifeOctober 20, 2022Iain M Hay, Katie E Mulholland, Tiffany Lai, Stephen C Graham, Hayley J Sharpe, Janet E DeaneLaboratories,Cat#610133,Western blot: 1:1000 Antibody,Anti-RFP (mouse monoclonal),Thermo Fisher Scientific,Cat#MA5-15257,Western blot: 1:1000 Antibody,Anti-Turbo-GFP (mouse monoclonal),OriGene,TA150041,Western blot: 1:1000 Antibody,Anti-Afadin

(14) ZIP1(+) fibroblasts protect lung cancer against chemotherapy via connexin-43 mediated intercellular Zn(2+) transferNature communicationsOctober 7, 2022Ni C, Lou X, Yao X, Wang L, Wan J, Duan X, Liang J, Zhang K, Yang Y, Zhang L, et al.MA1-26528; Invitrogen, USA), GFP (1:1000, clone GF28R, MA5-15256, Invitrogen). RFP (1:1000, clone RF5R, MA5-15257 Invitrogen), CX43 (1:1000, 26980-1-AP; Proteintech Group, China), S100A4 (1:1000, clone 3B11, kept in

(15) Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasisCell reportsSeptember 6, 2022Wei X, Zhang L, Zhang Y, Cooper C, Brewer C, Tsai CF, Wang YT, Glaz M, Wessells HB, Que J, et al.0315 Chicken anti-GFP,Abcam,Cat# ab13970; RRID: AB_300798 Mouse anti-RFP (Clone RF5R),Invitrogen,Cat# MA5–15257; RRID: AB_10999796 Rabbit anti-Mouse Vimentin,Cell Signaling Technology,Cat# 5741S; RRID: AB_10695459 Rat

(16) Differentiation Capacity of Bone Marrow-Derived Rat Mesenchymal Stem Cells from DsRed and Cre Transgenic Cre/loxP ModelsCellsSeptember 5, 2022Tseng HC, Wu MR, Lee CH, Hsiao JK.using skim milk and separately incubated overnight at 4 °C with primary antibodies against RFP (1:1000, MA5-15257, Thermo Fisher Scientific), Cre (1:1000, ab188568, Abcam), CDK4 (1:1000, MA5-12984, Thermo Fisher Scientific),

(17) Loss of Elp1 disrupts trigeminal ganglion neurodevelopment in a model of familial dysautonomiaeLifeJune 17, 2022Leonard CE, Quiros J, Lefcort F, Taneyhill LA.cat. AF567, 1:500), Pax3 (DSHB, ‘Pax3’, 1:200), BFABP (Sigma, ZRB13190-25ul, 1:500), and RFP (Thermo, MA515257, 1:400). All species/isotype-specific, Alexa Fluor-conjugated secondary antibodies were purchased from

(18) Progenitor potential of lung epithelial organoid cells in a transplantation modelCell ReportsApril 12, 2022Sharon M. Louie et al.,Santa Cruz Biotechnology,Cat# sc-7706; RRID:AB_2185507 Mouse monoclonal anti-RFP (RF5R),Invitrogen,Cat# MA5-15257; RRID:AB_10999796 Rabbit polyclonal anti-CC10 (FL-96),Santa Cruz Biotechnology,Cat# sc-25555; RRID:AB_2269914 Rat

(19) Dormant Nfatc1 reporter-marked basal stem/progenitor cells contribute to mammary lobuloalveoli formationiScienceMarch 18, 2022Ruiqi Liu et al.(Covance, 905501, 1:800), Ki67 (Abcam, Ab16667, 1:1000), NFAT2 (Abcam, Ab2796, 1:400), and RFP (Invitrogen, MA5-15257). Wholemount confocal imaging For wholemount confocal imaging, samples were dissected under a Leica

(20) Critical role for isoprenoids in apicoplast biogenesis by malaria parasiteseLifeMarch 8, 2022Okada M, Rajaram K, Swift RP, Mixon A, Maschek JA, Prigge ST, Sigala PA.Technologies P36931). PPS-RFP was visualized with a mouse monoclonal anti-RFP antibody (Thermo Fisher MA5-15257) and goat anti-mouse fluorescent 2° antibody (Invitrogen A11001). PPS-GFP was detected with a goat anti-GFP

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RFP Antibody from Thermo Fisher Scientific

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RFP Antibody from Thermo Fisher Scientific

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